Phase 1
Directed mutagenesis of wild type strain to produce a more salt tolerant strain.
Comparison CRISPR-Cas9 transformation efficiency to conventional homologous recombination. |
Phase 2Deletion of genes that transport salt out and allow water in.
Flip the direction of salt exporters so they become importers. Duplicate membrane synthesis and salt tolerances genes. |
Phase 3Designing system scale up and optimization.
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